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Cells were separated from muscle tissue debris by differential centrifugation and filtration (nylon cell strainers) prior to a final centrifugation step at 1500 x g for 3 min, then suspended in DMEM containing 10% normal horse serum (HS), 1% antibiotic-antimycotic mixture, and 0.5% gentamicin (DMEM-10% HS), and plated on poly-L-lysine and fibronectin-coated plates

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Khalil has received research support from Eli Lilly with no conflict of interest with this manuscript

The immunopeptidome of the senescent phenotype is associated with 70 peptides which makes senescent cells immunogenic and potentially targetable by immune cells

Rev Bras Reumatol 52(3):462 Kuo TR, Chen CH (2017) Bone biomarker for the clinical assessment of osteoporosis: recent developments and future perspectives